Volume 8, Issue 1 (6-2021)
nbr 2021, 8(1): 20-30 |
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Hanachi P, Sadeghi Ali Abadi H, Ghorbani N, Zarringhalami R, Kiarostami K. Evaluation of antioxidant and anticancer effects of Lavandula angustifolia and Melissa officinalis on HeLa, OVCAR-3 and MCF-7 cancer cell lines. nbr 2021; 8 (1) :20-30
URL: http://nbr.khu.ac.ir/article-1-3346-en.html
URL: http://nbr.khu.ac.ir/article-1-3346-en.html
Parichehr Hanachi * 
, Hojat Sadeghi Ali Abadi , Nasim Ghorbani , Roshanak Zarringhalami , Khadijeh Kiarostami
, Hojat Sadeghi Ali Abadi , Nasim Ghorbani , Roshanak Zarringhalami , Khadijeh Kiarostami
1Biotechnology Dep, Faculty of Biological Science, Alzahra University, Tehran Iran , P.hanachi@alzahra.ac.ir
Abstract: (3832 Views)
From ancient times, plants have been regarded as therapeutic agents, in addition to their usage as food. Plants are rich sources of antioxidant and phenolic compounds. Lavandula angustifolia and Melissa officinalis are medicinal herbs rich in antioxidant compounds. The aim of this study was to compare the antioxidant and anticancer properties of Lavandula angustifolia and Melissa officinalis extracts using aqueous, ethanol and methanol solvents, to select the best extraction methods and solvents and to evaluate the cytotoxic effect of the extracts on HeLa, OVCAR-3 and MCF-7 cancer cell lines. Methanol, aqueous and ethanol extracts were obtained from the dried leaves of the plants and the antioxidant activities of each extract were measured by DPPH and FRAP methods. Finally, the anticancer effects of the extracts on HeLa, OVCAR-3 and MCF-7 cancer cell lines were evaluated by MTT assay in order to identify the most efficient extract. Comparing the results of total antioxidant assay showed that the highest amount belonged to the ethanol extract of Melissa officinalis and Lavandula angustifolia methanol extracts using lyophilization method. The IC50 value of ethanol extract of Melissa officinalis was equal to 0.028 mg/ml on OVCAR-3 cells, which was the best result obtained in comparison with other solvents, and the ethanol extract of Lavandula angustifolia with IC50 = 2.07 mg/ml on MCF-7 cells was the most effective extract among the others. In HeLa cell-line, methanol extract of Lavandula angustifolia with IC50 = 7.36 mg/ml showed the highest cytotoxicity. In this study, for the first time, the effects of different extracts of Lavandula angustifolia and Melissa officinalis on MCF-7, OVCAR-3 and HeLa cancer cells were evaluated and the results showed that ethanol and methanol extracts of these plants had better toxic effect on cancer cells.
Type of Study: Original Article |
Subject:
Biotechnology
Received: 2020/02/27 | Revised: 2021/06/20 | Accepted: 2020/06/7 | Published: 2021/06/5 | ePublished: 2021/06/5
Received: 2020/02/27 | Revised: 2021/06/20 | Accepted: 2020/06/7 | Published: 2021/06/5 | ePublished: 2021/06/5
References
1. Abolfazl, K. 2009. Studying antioxidant activity of essential oil and extract of Iranian dendrochemistry. Science horizontal. Quarterly of Gonabad University of Medical Sciences & Health Services 15: 11-17.
2. Alizadeh Behbahani, B., Tabatabaei Yazdi, F., Mortazavi, A., Zendeboodi, F., Gholian, M. & Vasiee, A. 2013. Effect of aqueous and ethanolic extract of Eucalyptus camaldulensis L. on food infection and intoxication microorganisms "in vitro". Journal of Paramedical Sciences 4: 89-99 [DOI:10.29252/jnkums.6.1.99]
3. Azadmehr, A., Hajiaghaee, R., Rezazadeh, S., Afshari, A., Baradaran, B. & Ebrahimi, P. 2011. Evaluation of Lavandula officinalis extract on Lymphocyte proliferation and tumor necrosis factor-alpha production. Journal of Medicinal Plants 2: 142-147.
4. Chandrasekar, D., Madhusudhana, K., Ramakrishna, S. & Diwan, P. V. 2006. Determination of DPPH free radical scavenging activity by reversed-phase HPLC: a sensitive screening method for polyherbal formulations. Journal of Pharmaceutical and Biomedical Analysis 40: 460-464. [DOI:10.1016/j.jpba.2005.07.042]
5. Cozzani, S., Muselli, A., Desjobert, JM., Bernardini, AF. Tomi, F. & Casanova, J. 2005. Chemical composition of essential oil of Teucrium polium subsp. capitatum (L.) from Corsica. Flavour and Fragrance Journal 20: 436-441. [DOI:10.1002/ffj.1463]
6. Dorosti N, Zarabi S, Ahmadi S, Rostami R, Rashidi Pour M. 2017. Evaluation of anticancer activity methanolic extract of Allium Jesdianum and Nectaroscordum koelzi against HeLa and K562 cell lines. Yafte 19: 31-41.
7. Ebrahimzadeh, M. & Azad Bakhat, M. 2006. Pectin extraction and comparison of efficacy, degree of estrification and percentage of galacturonic acid in some citrus skin. Mazandaran University of Medical Sciences Journal 16: 52-59.
8. Elmore, S. 2007. Apoptosis: a review of programmed cell death. Toxicologic Pathology 35: 495-516. [DOI:10.1080/01926230701320337]
9. Hanachi, P., Zarringhalami, R. & Tamijani, R.R. 2018. Investigation of antioxidant properties of Polygonatum orientale Desf and Tilia dasystyla extracts by different methods and solvents. Hormozgan Medical Journal 22: 1-11. [DOI:10.5812/hmj.86504]
10. Hashemi, M., karami-Tehrani, F., Ghavami, S., Maddika, S. & Los, M. 2005. Adenosine and deoxyadenosine induces apoptosis in oestrogen receptor-positive and-negative human breast cancer cells via the intrinsic pathway. Cell Proliferation 38: 269-85. [DOI:10.1111/j.1365-2184.2005.00349.x]
11. Hosseini, S., Gharachurlou, M., Ghiyasi Tarzi, B., Qavami, B. 2014. Review of antioxidant capacity determination methods (Reaction Basis, Methods, Strengths and Weaknesses). Food Science and Nutrition, 11 (4): 89-111.
12. Hugh, D. & Mehmet, H. 2003. Cell proliferation and apoptosis. Advanced Methods Oxford: Bios Scientific Publishers Ltd 23:68.
13. Kauntz, H., Bousserouel, S., Gossé, F., & Raul, F. 2012. The flavonolignan silibinin potentiates TRAIL-induced apoptosis in human colon adenocarcinoma and in derived TRAIL-resistant metastatic cells. Apoptosis 17: 797-809. [DOI:10.1007/s10495-012-0731-4]
14. Mathew, S., & Abraham, T.E. 2006. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food and Chemical Toxicology 44: 198-206. [DOI:10.1016/j.fct.2005.06.013]
15. Momeny, M., Malehmir, M., Zakidizaji, M., Ghasemi, R., Ghadimi, H., Shokrgozar, M.A., Emami, A.H., Nafissi, S., Ghavamzadeh, A. & Ghaffari, S.H. 2010. Silibinin inhibits invasive properties of human glioblastoma U87MG cells through suppression of cathepsin B and nuclear factor kappa B-mediated induction of matrix metalloproteinase 9. Anti-Cancer Drugs 21: 252-60. [DOI:10.1097/CAD.0b013e3283340cd7]
16. Motalleb, G., Hanachi, P., Fauziah, O. & Asmah, R. 2008. Effect of Berberis vulgaris fruit extract on alpha-fetoprotein gene expression and chemical carcinogen metabolizing enzymes activities in hepatocarcinogenesis rats. Iranian Journal of Cancer Prevention 1: 33-44.
17. Sharopov, F.S., Wink, M., Khalifaev, D.R., Zhang, H., Dosoky, N.S. & Setzer, W.N. 2013. Composition and bioactivity of the essential oil of Melissa officinalis L. growing wild in Tajikistan. International Journal of Traditional and Natural Medicines 2: 86-96.
18. Zamanian-Azodi, M., Rezaie-Tavirani, M., Heydari-Kashal, S., Kalantari, S., Dailian, S. & Zali, H. 2012. Proteomics analysis of MKN45 cell line before and after treatment with Lavender aqueous extract. Gastroenterology and Hepatology from Bed to Bench 5: 35-42.
19. Zarringhalami, R., Hanachi, P., Kaya, E., Ağan, A. F., Ağan, K., & Donmez, M. 2020. Investigation of total phenolic content of Tilia dasystyla and Polygonatum orientale desf extracts and their cytotoxic effect on the osteogenic sarcoma (Saos-2) cancer cell line. International Journal of Cancer Management 13: e94130. [DOI:10.5812/ijcm.94130]
20. Zarringhalami, R., Hanachi, P., & Ramezani Tamijani, R. 2020. Cytotoxic effect of Tilia dasystyla and Polygonatum orientale Desf extracts on AGS and SKOV-3 cancer cell lines. Iranian Journal of Pharmaceutical Sciences 16: 9-16.
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